兔膝骨关节炎模型 z-bo 2020-10-26 385

　　Objective To prepare and evaluate a rabbit knee osteoarthritis model through rabbit cartilage defects.

　　Method The animals were randomly divided into 3 groups according to body weight and sex: sham operation group, model group and positive control glucosamine group, each with 12 animals. After 4 weeks of modeling, intragastric administration was started, once a day, for 4 consecutive weeks. After the last administration, the animals were sacrificed, the serum and synovial fluid levels of IL-1β and TNF-a were detected by ELISA kits, the articular cartilage and synovium were stained with HE, and the articular cartilage was scored by Makin's to evaluate its damage.

　　Results Compared with the sham operation group, the IL-1β and TNF-a levels in the serum and synovial fluid of the model group were significantly increased. Glucosamine can reduce the levels of IL-1β and TNF-a in the serum and synovial fluid. HE staining showed changes in cartilage structure and defects in the model group, and inflammatory cell infiltration in the synovium; cartilage score was significantly higher than that in the sham operation group. Glucosamine can reduce cartilage defects, reduce synovitis cell infiltration, and lower cartilage scores.

　　Conclusion Drilling articular cartilage can establish a rabbit knee osteoarthritis model, reflecting its main characteristics, and can reduce injury and inflammation by giving positive control drugs. This model can be used for the evaluation and screening of drugs for treating arthritis.