肝纤维化模型,四氯化碳 z-bo 2020-10-27 562

　　Objective To compare the effects of different doses of carbon tetrachloride on the inbred strain C57BL/6 mice liver fibrosis model in order to establish a stable liver fibrosis model.

　　Methods Five-week-old C57BL/6 inbred mice were selected and injected intraperitoneally with high, medium and low doses of carbon tetrachloride to induce liver fibrosis. After the experiment, blood and liver tissue were taken to detect plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST) levels and liver pathological changes.

　　Results After drug induction of inbred C57BL/6 mice, the animals in each dose group could survive up to 8 weeks. The survival rate of the high-dose group was only 20%, which was much lower than that of the middle and low groups; the degree of elevated serum transaminase levels and liver tissue Pathological changes have a dose-time effect relationship. Pathological analysis showed that hepatocyte degeneration/necrosis, mixed cell infiltration around central vein/portal area and liver fibrosis were seen in each dose group.

　　Conclusion 10% carbon tetrachloride administration can induce inbred C57BL/6 mice to form a stable liver fibrosis mouse model after 8 weeks, laying a foundation for subsequent liver fibrosis mechanism research and drug screening.