GFP裸小鼠,颅骨细胞 z-bo 2020-10-29 387

　　Objective The green fluorescent protein gene incoherent nude mice (GFP nude mice) established in this study played an important role in the tracking and study of the glioma microenvironment, but for the bones after autologous craniotomy. It also plays an important role in the treatment of absorption complications. There are no reports of impacts and mechanisms. This article aims to analyze the phenotype of GFP nude mouse skull cells and lay the foundation for preparing tool cells for skull resorption.

　　Methods After birth, 3dGFP nude mice were collected, the sterile bilateral parietal bones were dissected together with the periosteum, the skull was cut into small pieces of about 1 mm2, and placed in 1640 medium containing bovine fetal serum. In a carbon dioxide incubator used for short-term subculture, cells grown from bone slices were collected for related tests. The observation results of primary (P0) and secondary (P1, P2) cells show that the 90% transit time of the bottom of a 60 mm dish is about 6 to 8 days, and the number of sampled cells is about 2.3×106 to 2.5×106 , Means it is a cell /. The shape of the disc is mainly fibrous, with star-shaped and dendritic protrusions. All cells under a fluorescence microscope emit green fluorescence, and their shape is the same as that under a white light microscope. The detection of the labeled protein indicated that BMP-6 is also present in the entire cell population. Osteoblast precursor cells and CD206+, CD68+ macrophages.

　　Conclusion Based on the regeneration of the skull, in addition to osteoblast precursor cells as starting cells, macrophages must also participate in the maintenance of environmental homeostasis. Normally cultured P0, P1 and P2 third-generation cells are expected to be used as tool cells for further development. Used to study skull regeneration.