组织冰冻切片 z-bo 2020-11-02 498

　　Objective To explore the methods and techniques of making frozen sections of different tissues in experimental animal mice, improve the quality of frozen sections, and better serve the non-clinical safety evaluation of monoclonal antibodies.

　　Method Using the German Leica CM1950 thermostat cryostat, the heart, liver, spleen, lung, kidney, and brain tissues of healthy NIH mice were collected, embedded, frozen sectioned, HE stained, and mounted.

　　Results The sections were free of wrinkles, no knife marks, no damage, no excessive ice crystals in the tissue under the microscope, clear cell morphology, clear color of nucleus and cytoplasm, and good staining.

　　Conclusion When making frozen sections, strict control should be carried out in all aspects such as material extraction, embedding, quick freezing, temperature selection, and constant temperature frozen sectioning. However, different tissues will have differences according to their own characteristics and should be treated differently. In addition, while mastering the key technical points, we should also have a high degree of responsibility and patience, and produce high-quality frozen sections in order to better serve the non-clinical safety evaluation of monoclonal antibodies.