基因敲除小鼠 z-bo 2020-11-03 392

　　Objective To analyze the breeding and identification and adjustment methods of Ifnar knockout mice, and to provide an ideal animal model for the research of various viruses.

　　Methods The introduced homozygous Ifnar knockout mice were reared and reproduced in a cage with one male and two females. The tail genomic DNA was extracted from the offspring, and the target gene fragment was amplified by PCR and carried out by agarose gel electrophoresis. Judgment of genotype results.

　　Results Ifnar knockout mice were successfully bred and a batch of knockout mice were obtained. PCR method was used to successfully identify Ifnar knockout mice.