TRAP技术,急性肾损伤 z-bo 2020-11-03 402

　　Recently, USC stem cell scientists have successfully set up a TRAP mouse for acute kidney injury disease signals. Using this TRAP mouse can capture early signs of renal failure. The description is published in the Journal of Clinical Investigation. In the magazine. They used TRAP technology to generate new genetically modified mouse strains, extract cell and gene information from various solid organs of mice, and use it to determine acute kidney injury diseases.

　　The TRAP technology was invented by scientists from the Rockefeller Institute of Medicine in 2008. The TRAP technology attaches fluorescent labels to the ribosomes of the cell type of interest. Scientists can then collect the labeled ribosomes and determine which active genes "command" these ribosomes to produce protein. (TRAP stands for "Translation Ribosomal Affinity Purification")

　　On this basis, Jing Liu, a researcher at the University of Southern California and others, designed a simpler and more convenient technique for capturing mice with disease signals. When any one of the thousands of existing genetically modified mouse strains mates with disease signal-trapping mice and breeds, they can produce offspring, and specific organs or cell types in these offspring mice carry markers Ribosome.

　　Liu and her colleagues used disease signal capture mice to label four different types of kidney cells and determine the early signs of acute kidney injury. Due to surgery, infection, or drug toxicity, 5%-7% of all hospitalized patients have experienced acute kidney injury, leading to chronic kidney disease or death.

　　Currently, doctors can detect acute kidney injury only one full day after acute kidney injury occurs. Disease signal capture mice can detect disease signals early, and this will greatly increase the probability of the patient's final diagnosis of the disease.