非酒精性脂肪性肝 z-bo 2020-11-06 332

　　Objective: To study the dynamic changes and significance of oxidative stress and cytokines in the formation of non-alcoholic fatty liver disease (NAFLD) in gerbils.

　　Methods: Forty-eight male gerbils were randomly divided into normal group and model group, 24 in each group, fed with normal diet and high-fat diet, and treated in each group on the 4th, 8th and 16th weekend of the experiment. 8 gerbils, dynamic detection of liver tissue homogenate MDA content and SOD, GSH-PX and T-AOC activity by chemical method, liquid suspension chip technology dynamic detection of serum TNF-α, INF-γ, IL-10 and other cytokine levels .

　　Results: With the progress of NAFLD disease, the content of MDA in the liver tissue of gerbils gradually increased, and were significantly higher than the normal group (P<0.01); the T-AOC level first increased slightly and then decreased, compared with the normal group at 4 and 16 weeks Significant decrease (P<0.05); SOD level first increased significantly and then decreased significantly, at 4 weeks, it was significantly higher than the normal group (P<0.05), and at 8 weeks and 16 weeks, it was significantly decreased (P<0.05, P<0.01); GSH -PX level showed a gradual downward trend, significantly lower than the normal group at 8 weeks and 16 weeks (P<0.05). Serum cytokines TNF-α and IFN-γ gradually increased with the progress of NAFLD, while IL-10 levels gradually decreased, which were statistically different from the normal group at 8 weeks and 16 weeks (P<0.05, P<0.01).

　　Conclusion: The oxidative stress-related indicators and inflammatory cytokines of the NAFLD model of Mongolian gerbils induced by high fat change significantly with the development of simple fatty liver to steatohepatitis, hepatic fibrosis and cirrhosis, and they participate in the occurrence and development of NAFLD .