凝血四项,SD大鼠标本 z-bo 2020-11-06 358

　　Objective: To explore the best anticoagulation ratio in rats, and analyze the influence of insufficient blood volume of specimens on the four test results of coagulation.

　　Method: 60 rats were divided into 2 groups, and blood was collected from the abdominal aorta on an empty stomach for 12 h according to the vacuum blood collection method. The first group of 20 animals was used for whole blood cell determination. The automatic blood cell counter detects hematocrit (HCT) and platelet count (platelet, PLT). The second group of 40 rats, each mouse collected 2 tubes of blood, according to the anticoagulation ratio [ratio of sodium citrate anticoagulant to whole blood (V:V)] divided into 1:9 (control group) and 1:5 ( Experimental group), 1:8 (experimental group) and 1:7 (experimental group), centrifuged to obtain platelet-poor plasma. Automatic blood coagulation analyzer detects four blood coagulation items: prothrombin time (PT), activated partial thromboplatin time (APTT), thrombin time (TT), fibrinogen (Fibrinogen, FIB).

　　Results: SD rats HCT (%) is 41.7 ± 2.9, PLT (×109/L) is 1114 ± 173. With the increase of anticoagulant ratio, PT, APTT, TT prolonged and FIB decreased. Compared with the control group: 1:8 experimental group, the difference was not statistically significant. In the 1:7 experimental group, except for the statistically significant difference in TT, the differences in the other three indicators were not statistically significant. 1:5 experimental group, the difference is statistically significant.

　　Conclusion: The results of the four coagulation items are affected by the anticoagulation ratio. 1:9 is the best anticoagulant ratio in rats, and 1:8 is acceptable. Rats have their unique physiological characteristics. It provides basic scientific data and basic theoretical basis for rat related research.