小鼠实验,帕金森症 z-bo 2020-11-10 410

　　The Department of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, through cooperation with Shanghai University of Traditional Chinese Medicine Institute of Acupuncture and Meridian, School of Acupuncture and Tuina, and Department of Acupuncture and Moxibustion of Longhua Hospital, explored acupuncture on a mouse model of Pakinson's disease Molecular mechanism of action. Studies have found that acupuncture at the Yanglingquan point in the legs of mice can regulate the autophagy pathway of dopaminergic neurons in the brain through a non-mTOR dependent pathway, restore their damaged autophagy levels, and clear α-synaptic nuclei Protein (α-synuclein) accumulates in the brain and significantly improves its exercise capacity.

　　Acupuncture therapy has a history of thousands of years in our country. It uses acupuncture or moxibustion at specific points to achieve the purpose of disease prevention and treatment. At present, acupuncture has begun to gain more and more widespread recognition in the international medical community, and the research on its molecular mechanism has also attracted widespread attention. For example, American researchers have revealed the molecular mechanisms of acupuncture analgesia and electroacupuncture anti-inflammatory (Goldman et al. Nature Neuroscience, 2010, 13, 883; Torres-Rosas et al. Nature Medicine, 2014, 20, 291). Neurodegenerative diseases (Parkinson's disease, Alzheimer's disease, Huntington's disease, etc.) are a class of diseases that pose a major threat to humans. Acupuncture has a clear effect on relieving symptoms, but its molecular mechanism is not clear.

　　Autophagy is an important process for the turnover of intracellular substances in eukaryotes, and it is highly conserved in evolution. Studies have shown that autophagy pathway disorders are related to the occurrence and development of many diseases, including neurodegenerative diseases. In recent years, some work has also proved that exercise and mechanical stimulation can play a role by regulating the autophagy level of cells and the body (for example, He et al. Nature, 2012, 481, 511).

　　Inspired by this, researchers Fan Chunhai, Hu Jun and Associate Researcher Sun Yanhong of Shanghai Institute of Applied Physics and Shanghai University of Traditional Chinese Medicine Professor Wu Huangan and Pei Jian formed a cooperative research team to explore whether acupuncture, as a special mechanical stimulation, can regulate the body’s The level of autophagy plays a role in the treatment of neurodegenerative diseases. Tian Tian and other researchers investigated the changes of autophagy-related indexes in the brain before and after acupuncture in Parkinson's disease model mice. Studies have shown that acupuncture at Yanglingquan point can significantly up-regulate the expression of lysosomal membrane protein of dopaminergic neurons in the mouse brain and repair the blocked autophagy-lysosomal pathway. The up-regulation of autophagy inhibited the accumulation of α-synuclein, which is the hallmark pathological protein of Parkinson's disease, in the mouse brain. With the restoration of autophagy levels, the damage of dopaminergic neurons in mice was improved, and the vitality of dopaminergic neurons and motor behavior ability were significantly improved. Further studies have shown that this up-regulation of autophagy induced by acupuncture does not depend on the mTOR pathway. This feature is different from the commonly used autophagy inducers (such as rapamycin, rapamycin), and as a physical therapy, acupuncture provides a new way to avoid the side effects of chemical drugs and avoid the blood-brain barrier problem in chemical drug delivery. may. Therefore, this study not only explored the molecular mechanism of acupuncture and moxibustion in improving neurodegenerative diseases from the perspective of autophagy, but also provided useful ideas for the application of acupuncture and moxibustion in neurodegenerative diseases and aging.