银杏叶提取物,帕金森 z-bo 2020-11-10 331

　　Objective: To observe the effects of ginkgo biloba extract (GBE) on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) inducing Parkinson's disease at different ages. PD) Prevention and treatment of mice.

　　Methods: 60 C57BL/6 mice of 8 weeks and 32 weeks of age were randomly divided into negative group, model group, high, medium, and low dose GBE administration group and positive group. The PD animal model was prepared by intraperitoneal injection of MPTP. , Ginkgo biloba extract is used for intragastric treatment. Climbing, hanging and swimming experiments were used to detect the motor function of mouse limbs, and the activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondioxide in mouse brain tissue was measured by spectrophotometry. Aldehyde (MDA) content, immunohistochemical method was used to observe the changes of the number of positive cells of substantia nigra tyrosine hydroxylase (TH) and striatal dopamine transporter (DAT) in mice.

　　Results: (1) Compared with the negative control group, the motor function score, brain GSH-Px activity, substantia nigra TH-positive cells and striatal DAT-positive cells of the 8-week-old model group were significantly decreased, and the brain tissue The MDA content increased significantly, indicating that the PD model was successfully replicated. Compared with the model group, the motor function score, SOD and GSH-Px activities, and the average optical density of TH-positive cells in the substantia nigra of the 8-week-old mice in the Ginkgo biloba extract administration group increased significantly, while the MDA content decreased significantly. (2) Compared with the negative control group, the 32-week-old model group mice's motor function score, substantia nigra TH-positive cells and striatum DAT-positive cells average optical density values all significantly decreased, SOD and GSH-Px activities decreased, brain tissue The increase of MDA content indicates that the model is successfully copied. Compared with the model group, 32-week-old mice in the Ginkgo biloba extract-administered group showed significant increases in motor function scores, SOD activity, substantia nigra TH and DAT positive cells in the striatum, and significantly decreased brain MDA content.

　　Conclusion: Ginkgo biloba extract has a certain preventive effect on PD mice of different ages induced by MPTP.