雷帕霉素,主动脉瓣膜间质细胞 z-bo 2020-11-11 353

　　OBJECTIVE: To target rapamycin to inhibit mTOR and explore its effect on the proliferation of rat heart valve interstitial cells.

　　Methods: The rat valve interstitial cells were isolated in vitro and cultured and identified; the cells were randomly divided into two groups: rapamycin group and control group; MTT method was used to detect and draw the growth curve of rat valve interstitial cells before and after treatment; Cytometry was used to detect the effect of rapamycin on the cell cycle; RT-PCR was used to detect the mRNA expression levels of S6 and P70S6K in cells; Western blotting was used to detect the expression levels of S6, P70S6K, P-S6, and P-P70S6K proteins in cells.

　　Result: The rat valve interstitial cells isolated and cultured in vitro were successfully isolated and cultured; the cell activity of the control group was significantly higher than the cells of the drug-added group (P<0.05), and the growth of the cells in the drug-added group slowed down. Flow cytometry showed that there was no significant difference in the proportion of cells in each cycle between the drug-added group and the control group; the phosphorylation levels of S6 protein and P70S6K protein in the drug-added group decreased (P<0.05).

　　Conclusion: Rapamycin can inhibit the proliferation of valvular interstitial cells. The reason may not be caused by changing the cell cycle but by inhibiting mTOR and down-regulating the phosphorylation level of its target proteins S6 and P70S6K.