慢性皮质酮 z-bo 2020-11-12 368

　　Purpose: To establish a drinking method, chronically give corticosterone, and observe the effect of corticosterone on learning and memory and synapse-related proteins.

　　Method: c57BL/6J male mice were randomly divided into normal group and corticosterone group, with 10 mice in each group. The corticosterone group drank 0.45% hydroxypropyl-β-cyclodextrin solution containing corticosterone, 5 mg/(kg·d). The animals in the normal group were given 0.45% hydroxypropyl-β-cyclodextrin solution. After 35 days of model building, social recognition experiments and water maze experiments were carried out. After the behavioral experiment, the animal was decapitated and the hippocampus was taken, and the synapse-related protein was determined by western blot.

　　Result: The social recognition experiment showed that the sniffing time of the normal group and the corticosterone group to the stimulated cage was longer than that of the empty cage (P<0.05). The sniffing time of the control group to stimulate mouse 2 was longer than that of stimulate mouse 1 (P <0.05). There was no significant difference between the sniffing time of corticosterone group on stimulating mouse 2 and stimulating mouse 1. The navigation results of the water maze experiment showed that the learning ability of the corticosterone group was decreased, and the latency of seeking platform was prolonged (P<0.05). The results of space exploration showed that the number of crossings, the number of times to enter the real quadrant and the distance to the real quadrant in the corticosterone group were significantly reduced (P <0.05, P <0.05, P <0.05). Western blot results showed that in the corticosterone group, postsynaptic density (PSD-95), synapsin (synapsin-1, Syn-1) and phosphorylated extracellular signal-regulated kinase (ERK) ) Expression decreased significantly (P <0.01, P <0.01, P <0.05).

　　Conclusion: By drinking and chronically administering corticosterone, the animals have social memory and spatial learning and memory disorders, which may be related to the decreased expression of hippocampal synaptic-related proteins and impaired synaptic function.