16S rRNA高通量测序法,实验动物,口腔菌群 z-bo 2020-11-13 415

　　Objective: To use 16S rRNA high-throughput sequencing method to determine the oral flora of several commonly used experimental animals (Tibet minipigs, beagle dogs, macaques, New Zealand rabbits, Wistar rats), and to compare and analyze the oral flora with humans. Provide basic data for the study of oral microecology animal models.

　　Method: Use disposable cotton swabs to collect oral flora specimens from Tibet miniature pigs, beagle dogs, macaques, New Zealand rabbits, Wistar rats and humans, extract the total DNA of the samples, and amplify the 16S rRNA V4 region using labeled universal primers The fragments were sequenced by Illumina and analyzed by BIPES and QⅡME to compare the diversity and structure of the flora.

　　Result: The abundance of oral flora between humans and five commonly used experimental animals is significantly different (P<0.05). Different types of animals have their own unique oral flora, but the oral flora of monkeys is most similar to that of humans.

　　Conclusion: According to the degree of similarity with a certain type of human oral flora, among the five animal species, the levels of Fusobacterium and Porphyromonas in the monkey oral cavity are the most similar to those of humans, suggesting that monkeys may be the study of human oral bacteria Group of more suitable model animals. From the perspective of specific bacteria phyla, Tibet minipig may be a more suitable model animal for studying diseases related to Proteobacteria; Beagle may be a more suitable model animal for studying diseases related to Spirochaetes.