糖尿病大鼠,抗氧化剂治疗 z-bo 2020-11-13 335

　　Objective: To explore the changes in SOD activity and protein expression levels in the main organs of diabetic rats, and to observe the effect of the antioxidant N-acetylcysteine (NAC) on the body's antioxidant status after short-term treatment (4 weeks) .

　　Methods: STZ-induced diabetic rats (group D, n=8) were given NAC 1.5 g/kg per day (D+N group, n=8), and normal control group (group C, n=8) were given simultaneously Same volume of saline. After 4 weeks, the heart, lung, liver, and kidney tissues were obtained, and the kit was used to detect plasma total SOD, total antioxidant concentration, lipid peroxidation specific marker 15-F2t-isoprostane, and total superoxide dismutase ( Superoxide dismutase (SOD) activity, Western blotting analysis SOD subtype Cu/Zn-SOD and Mn-SOD protein expression levels.

　　Results: Compared with group C, the plasma 15-F2t-isoprostane, total antioxidant concentration and total SOD activity in myocardial tissue of rats in group D increased significantly, while total SOD activity in plasma, lung, liver, and kidney tissues decreased significantly; The expression level of Cu/Zn-SOD protein in heart and lung tissues was significantly increased, while that in liver and kidney tissues was significantly decreased; the expression level of Mn-SOD protein in lung and kidney tissues was significantly decreased, while the expression of Mn-SOD protein in liver tissues was significantly increased, but myocardial tissue The change is not obvious. NAC intervention can reverse the above changes to varying degrees, but further reduce the expression of Mn-SOD in kidney tissue.

　　Conclusion: The total SOD activity, Cu/Zn-SOD and Mn-SOD protein expression levels in the various tissues of diabetic rats have tissue differences. The antioxidant NAC can restore the antioxidant levels in various tissues of diabetic rats to prevent or Delay the function of target organ damage related to diabetes.