上皮钠离子 z-bo 2020-11-13 354

　　OBJECTIVE: This article explores the effect of the epithelial sodium ion channel (ENaC) on the function and activity of osteoclasts.

　　Method: Rat macrophage colony stimulating factor and nuclear transcription factor-κB receptor activator ligand were used to induce rat bone marrow monocytes to differentiate into osteoclasts. Inoculate a 12-well plate at a density of 1.5×104, and check the random table to divide 3 wells into 1 group, divided into 4 groups: Control group and different concentration of amiloride (Ami, ENaC inhibitor) group. Tartrate-resistant acid phosphatase (TRAP) staining was used to identify positive osteoclasts; osteoclasts and bone fragments were co-cultured to determine the number of bone resorption lacunas; RT-PCR was used to analyze the osteoclast marker enzyme gene cathepsin K ( CK) expression.

　　Result: After treating osteoclasts with different concentrations of Ami, TRAP staining-positive osteoclasts decreased, inhibited the formation of osteoclasts and bone resorption, and reduced the expression of osteoclast-specific gene CK.

　　Conclusion: This experiment demonstrated the expression of ENaC on osteoclasts at the cellular level and regulates the differentiation and bone resorption of osteoclasts, indicating that ENaC may be involved in the functional regulation of osteoclasts, suggesting that osteoclasts may have a regulation related to ENaC This new approach provides a new idea for the study of bone metabolism.