实验动物 z-bo 2020-07-29 671

　　After preparing for all animal experiments, order the required experimental animals with corresponding quality certificates from the experimental animal supply unit according to the determined number of experimental animals. When purchasing, you should immediately obtain the exact name of the breed, strain and offspring that match your animal from the supplier. Genetic background or source, microbial status, qualification, signature of breeding unit manager. Laboratory animals without the above data shall not be used.

　　Under normal circumstances, the supplier will provide the corresponding animal transport container and pack the animal. Regardless of the transportation distance, you should strive to keep the animal healthy and prevent it from being contaminated or suffocated to death. The transportation container should be selected according to the animal's microbial level, and the size of the container should enable the animal to have normal posture and movement. Normal animals can be transported in cages, but animals above the clean level are usually transported in cardboard boxes with filter paper windows, while sterile animals are transported in special metal sterilization boxes. During transportation, external temperature and humidity can have a significant impact on animals. If you do not have air-conditioned transportation, we will try to avoid summer and summer heat by choosing the season with small temperature difference between indoor and outdoor (air transportation), and transport animals. use). There are certain difficulties in watering animals during transportation, but usually the transportation time without feeding and watering is 5 to 6 hours. Watering can only be done within 24 hours and must be watered for more than 24 hours. Have. Normal animals can use potatoes, fresh fruits or vegetables instead of direct water supply. For animals above the clean level, 2% to 3% agar protein ept water is usually used. Alternatively, the apple can be immersed in a 2% sodium hypochlorite solution and placed in the transfer window after 30 minutes of irradiation. Go through the obstacle area and immediately rinse the apple with drinking water and place it in the transport cage with the ingredients.

　　After purchasing a human animal and returning to this unit, you will not be able to enter the animal laboratory directly. You must first check and quarantine in the "quarantine area". Acceptance inspection to see whether the animals meet the order requirements, find and remove abnormal animals. Etiological examination should be performed as much as possible. During the inspection and acceptance period, the animals are separated from the males and females and kept in separate cages for a period of time. This allows the animal to regain strength and adapt to the new environment and management.

　　Before grouping, animals must be weighed. The animals should be weighed before feeding. Weigh the animals when they are relatively stable, because the animals are timid and fearful, and can move easily when weighing. The scale used for weighing must be calibrated before use, and each weighing must be fixed at the same time. Different animals have different weighing requirements and must be selected correctly.