rhPTH(1-34),骨折愈合 z-bo 2020-11-13 445

　　OBJECTIVE: To observe the effect of single subcutaneous injection of parathyroid hormone rhPTH (1-34) every week on promoting fracture healing in rats.

　　Methods: Fifty 3-month-old male SD rats with left tibia fractures, after Kirschner wire internal fixation, were randomly divided into 5 groups (n=10/group) according to the dose and frequency of subcutaneous injection of rhPTH (1-34) : Weekly 10, 20 μg/kg group; daily 10, 20 μg/kg group; weekly subcutaneous injection of the same amount (500 μL) of saline as the control group. After 4 weeks of treatment, they were sacrificed and collected materials. Small animal X-rays, dual-energy X-rays, micro-CT and three-point bending biomechanical experiments were performed to detect fracture healing.

　　Results: The bone mineral density of rhPTH (1-34) injected subcutaneously every week or daily was higher than that of the control group (P<0.05); the fracture healing effect of the 20 μg/kg group per week was better than that of the control group. Among them, rhPTH (1-34) ) The 20 μg/kg weekly group was 26.2% and 51.4% higher than the control group in terms of bone density, mineralized callus volume, total callus volume, mineralized callus volume as a percentage of total callus volume, and maximum load. , 21.6%, 24.5%, 29.3%. The maximum load of 20 μg/kg per week, 10 μg/kg per day and 20 μg/kg per day was higher than that of the control group (P<0.01); 10="" 20="" p="">0.05 per week).

　　Conclusion: Weekly subcutaneous injection of parathyroid hormone (rhPTH1-34) (10, 20 μg/kg) can promote fracture healing, but the same single dose, daily subcutaneous injection is still better than weekly subcutaneous effect.