星形胶质细胞肿胀,谷氨酸 z-bo 2020-11-13 302

　　OBJECTIVE: To compare the differences in the swelling of glutamate-induced astrocytes (AST) between Wistar rats and SD rats.

　　Methods: Wistar rats and SD rats of 1 day old were used for primary and subculture of AST. After subculture for 10 days, they were incubated with 1, 10 mmol/L glutamic acid for 48 hours, and the cells were detected by the release rate of lactate dehydrogenase. The activity is measured by glial fibrillary acidic protein (GFAP) immunofluorescence staining and Image Pro Plus software is used to measure cell perimeter to indicate cell volume changes, and reverse transcription real-time fluorescent quantitative PCR method to detect changes in aquaporin 4 (AQP4) mRNA expression.

　　Result: There is no difference in the effect of glutamate on the AST activity of different species of rats (P>0.05). Under normal circumstances, the AST circumference of Wistar rats is smaller than that of SD rats, and after treatment with glutamate, it is significantly larger than that of SD rats (P<0.05). After administration of 1 mmol/L glutamate, the AQP4 mRNA expression of AST in Wistar rats was significantly higher than that in SD rats (P<0.05).

　　Conclusion: Wistar rat AST has more obvious cell swelling caused by glutamate than SD rats, and there is a difference in the effect of glutamate on the change of AQP4 expression on AST of two species of rats.