脐血间充质干细胞移植,谷氨酰胺,大鼠肠缺血再灌注损伤 z-bo 2020-11-13 432

　　Objective: To investigate the effect of glutamine combined with cord blood mesenchymal stem cells (MSCs) transplantation on intestinal ischemia-reperfusion injury in rats.

　　Method: Resuscitate and culture cord blood mesenchymal stem cells in vitro before transplantation, and observe the whereabouts of cord blood mesenchymal stem cells after CM-DiI fluorescent labeling. 80 SD rats were randomly divided into normal control group, ischemia-reperfusion injury group, glutamine group, MSCs transplantation group and combination group with 15 rats in each group. Control group received normal saline enema, injury group received TNB (S ethanol dilution) enema, 1 h after TNBS modeling, glutamine group received 0.45 g/kg glutamine in the tail vein, and MSCs transplantation group received 1 h in the tail vein. ×1010/L umbilical cord blood mesenchymal stem cell suspension, the combined group received 0.45 g/kg glutamine into the tail vein + cord blood mesenchymal stem cell suspension 1×1010/L. The levels of intestinal fatty acid binding protein (IFABP), interleukin-6 (IL-6) and superoxide dismutase (SOD) in the serum of each group of rats were detected by ELISA; each group was detected 1 h and 3 h after reperfusion Water content of intestinal tissue; the expression of mRNA and protein of caspase-3, NF-kB, and Bcl-2 in rat intestinal mucosal epithelial cells after transplantation of glutamine combined with MSCs was observed by RT-PCR and Western blot.

　　Result: The transplanted MSCs cells were observed to be distributed in the intestinal mucosal lymphoid tissue and glandular epithelial cells by fluorescence tracing, indicating that MSCs may be involved in the repair process of intestinal ischemia-reperfusion injury. Comparison of the changes in serum SOD, IFABP, and IL-6 levels of rats in each group. The serum levels of IFABP and IL-6 in the injury group were significantly higher than those in the control group, while the glutamine group, MSCs transplantation group and combination group were compared Compared with the control group, the SOD content in the serum of the injury group was significantly lower than that of the control group, while the glutamine group, the MSCs transplantation group and the combination group were significantly higher than that in the combination group. Obviously (P<0.05). 1="" 3="" reperfusion="" p="">0.05). Compared with the control group, the mRNA and protein expressions of caspase-3 and NF-kB in the intestinal mucosal epithelial cells of the injured group were significantly up-regulated, and the mRNA and protein expressions of Bcl-2 were significantly down-regulated (P<0.05), while the glutamine group and MSCs transplantation Compared with the group and the combination group, the mRNA and protein expression of caspase-3 and NF-kB were significantly down-regulated, and the mRNA and protein expression of Bcl-2 were significantly up-regulated (P<0.05). there="" was="" no="" difference="" between="" the="" glutamine="" group="" and="" mscs="" transplantation="" group.="" a="" statistical="" p="">0.05), but there was a significant difference between the two groups and the combined group (P<0.05).

　　Conclusion: After transplantation of glutamine group and MSCs, the degree of intestinal ischemia-reperfusion injury in rats was significantly reduced. It may reduce the intestinal mucosal ischemia-reperfusion injury by inhibiting the expression of caspase-3 and NF-kB and promoting the expression of Bcl-2 .