干细胞,FGF-2/PELA/BMP-2微囊支架 z-bo 2020-11-16 313

　　OBJECTIVE: To observe the effects of fibroblast growth factor-2/polylactic acid-polyethylene glycol-polylactic acid/bone morphogenetic protein-2 (FGF-2/PELA/BMP-2) microcapsule scaffold on the growth of SD rat periosteum-derived stem cells Bone differentiation.

　　Methods: Extracting SD rat periosteal stem cells (PDSC), performing flow cytometric identification of surface markers and induction of osteogenesis, cartilage, and adipogenesis, followed by Alizarin Red, Toluidine Blue, Alcian Blue, and Oil Red O staining and immunofluorescence experiment. Four groups of materials, FGF-2/PELA/BMP-2, FGF-2/PELA, PELA/BMP-2, and PELA were prepared, and the surface of the microcapsules were observed by scanning electron microscopy (SEM). The controlled release curve of the two factors was made by ELISA experiment. The 4 groups of material extracts were co-cultured with the third-generation periosteal-derived stem cells, and alkaline phosphatase (AKP) activity was detected on 7 and 14 days, and qRT-PCR osteogenic genes were performed on 7, 14, and 21 days. Expression detection, observation and comparison of the osteogenic differentiation ability of each group of PDSC, statistical analysis after data collection.

　　Result: Flow cytometry surface marker identification showed that PDSC expressed mesenchymal stem cell surface markers, and the three-line induced differentiation staining showed that PDSC has multidirectional differentiation capabilities such as osteogenic, cartilage, and adipogenesis. The results of AKP activity showed that the FGF-2/PELA/BMP-2 group had the highest AKP activity on the 7th and 14th day of PDSC culture, with significant differences. The qRT-PCR results of the FGF-2/PELA/BMP-2 group indicated that the expression of RunX-2 and OCN were higher than those of other groups, and the expression of RunX-2 reached the peak on the 14th day, and the OCN showed a continuous increase trend.

　　Conclusion: The cytokine activity in the FGF-2/PELA/BMP-2 biomimetic controlled-release microcapsule scaffold is retained, and it has a higher ability to promote osteogenic differentiation of PDSC than other experimental groups.