2型糖尿病神经痛 z-bo 2020-11-16 407

　　Objective: To observe the effect of low-frequency electroacupuncture (EA) on the pain threshold of type 2 diabetic neuropathic pain (DNP) model rats and the expression of P2X3 receptor in the L5 dorsal root ganglion (DRG) .

　　Method: Experiment 1: 50 SD rats were randomly divided into 8 control groups and 42 model groups. The model was given a high-fat and high-sugar diet combined with a small dose of streptozotocin (STZ, 35 mg/kg) intraperitoneally to establish a rat DNP model. The control group was fed with regular feed and given the same dose of citrate buffer injection. Rats with successful models in the model building are further divided into a model group (DNP group) and a low-frequency electroacupuncture treatment group (DNP+EA group). Electroacupuncture treatment was performed on bilateral "Zusanli" and "Kunlun" points, with a frequency of 2 Hz and intensity of 1 mA for 15 minutes, followed by 2 mA for 15 minutes, once a day, for a total of 7 treatments. Observe the changes in insulin sensitivity index (ISI) and fasting plasma glucose (FPG) levels of rats fed high-fat and high-sugar for 0, 5 weeks and 0, 5, and 7 weeks. The changes of paw withdrawal thresholds (PWTs) of rats fed high-fat and high-sugar for 0, 5, 7 weeks and electroacupuncture at 3, 5, and 7 d 6 time points; immunofluorescence method was used to determine L5 DRG P2X3 receptor Body expression. Experiment 2: Divide the rats successfully modeled by DNP into electroacupuncture group (EA+Vehicle group) and P2X3 agonist group (EA+αβ-meATP group). Electroacupuncture intervention is the same as above. The EA+αβ-meATP group was injected with αβ-meATP (0.6 μmol/L, 100 μL) under the toes of rats before each electroacupuncture intervention. EA+vehicle group rats were injected with the same dose of PBS buffer, and the other interventions were the same. Detect mechanical pain threshold.

　　Results: ①Compared with the control group, the ISI of the model group was significantly reduced after high-fat and high-sugar feeding for 5 weeks (P<0.01), and the FPG was significantly increased after high-fat and high-sugar feeding for 7 weeks (P<0.01). It shows that the type 2 diabetes model was successfully established (the success rate of model building was 69.04%); ②PWTs: Compared with the control group, the bilateral PWTs of rats in the model group were significantly reduced (P<0.01), indicating that the type 2 DNP model was successful; and the model group In comparison, rats in the low-frequency electroacupuncture treatment group showed a significant increase in bilateral PWTs at each time point after treatment (P<0.01); compared with the electroacupuncture group, the bilateral PWTs in the P2X3 agonist group were significantly reduced (P<0.01) ). ③The results of immunofluorescence method showed that: Compared with the control group, the expression of L5 DRG P2X3 positive cells in the model group increased significantly (P<0.01); compared with the model group, the expression of L5 DRG P2X3 positive cells in the low-frequency electroacupuncture treatment group was all Significantly reduced (P<0.01).

　　Conclusion: Low-frequency electroacupuncture can effectively improve type 2 DNP by down-regulating the L5 DRG P2X3 receptor.