人羊膜间充质干细胞,慢性酒精性肝 z-bo 2020-11-16 384

　　Purpose: Studies have shown that human amniotic mesenchymal stem cells (hAMSCs) have a variety of differentiation and proliferation functions, which can induce differentiation into hepatocyte-like cells, thereby having a certain repair effect on liver injury.

　　Methods: In vitro resuscitation and culture of human amniotic membrane mesenchymal stem cells, 66 healthy female Wistar rats, 22 were randomly selected without any treatment as normal control group, and the remaining 44 rats were given chronic alcohol by gavage with white wine for 30 days After modeling, they were randomly divided into model group (1 mL PBS injected into tail vein), hAMSCs group (1 mL hAMSCs (2×106 pieces) injected through tail vein, 22 in each group. After transplantation For 4 weeks, the automatic biochemical analyzer was used to detect serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), serum total bilirubin (TBIL), and serum albumin ( ALB), total serum protein (Tp); simultaneous detection of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), catalase (CAT), and Changes in the contents of malondialdehyde (MDA) and IL-4; the distribution of hAMSCs labeled with PKH-26 was observed by immunofluorescence microscope, and the apoptosis of liver cells in each group was detected by TUNEL method.

　　Results: The blood biochemical test results of rats in each group showed that compared with the normal control group, the contents of ALT, AST, TBIL, and Tp in the model group were significantly increased. Compared with the model group, the levels of ALT, AST, TBIL, and Tp in the hAMSCs transplantation group were significantly increased. Tp content decreased significantly (P<0.05). Compared with the normal control group, the ALB content in the model group was significantly decreased, while compared with the model group, the ALB content in the hAMSCs transplantation group was significantly increased (P<0.05); compared with the model group The contents of SOD, GSH-PX, and CAT in the hAMSCs transplantation group were significantly increased, and the contents of MDA and IL-4 were significantly decreased. Compared with the normal control group, the contents of SOD, GSH-PX, and CAT in the hAMSCs transplantation group were all decreased, and the contents of MDA and IL- The contents of 4 were all increased (P<0.05); the transplanted PKH-26-labeled positive hAMSCs cells were found to be distributed in the liver tissue in the transplantation group, and no such cell distribution was found in the other groups (P<0.05); TUNEL method detected each group The number of apoptotic cells was seen in hepatocyte apoptosis. The model group was the most (34.27±5.71), followed by the hAMSCs transplantation group (18.42±3.95). No apoptotic cells were seen in the normal control group.

　　Conclusion: Transplantation of human amniotic membrane mesenchymal stem cells can improve the blood biochemical indicators of liver cirrhosis in rats, and hAMSCs transplantation can reduce liver cell apoptosis in rats with chronic alcoholic liver injury.