大鼠脑缺血再灌注损伤 z-bo 2020-11-17 342

　　Objective: To investigate the effect of hydrogen-rich liquid on hippocampal neuron apoptosis and PI3K/Akt/FoxO1 signal pathway expression induced by cerebral ischemia-reperfusion in rats.

　　Methods: The middle cerebral artery of the rat was blocked by the suture method, and the focal cerebral ischemia/reperfusion (I/R) model was established. SD rats were randomly divided into sham operation group (Sham group), ischemia/reperfusion group Perfusion group (I/R group) and hydrogen-rich liquid treatment group (HRS group), each with 10 animals; 24 hours after reperfusion in each group, serum inflammatory factors IL-6, TNF-α and IL-1β were detected by ELISA The changes in hippocampus were observed by HE staining, the apoptosis of brain tissue was observed by TUNEL method, and the expression changes of hippocampal p-PI3K, Akt, caspase-3 and FoxO1 protein were detected by Western blot.

　　Results: Compared with the Sham group, the pyramidal cells of the I/R group were loosely arranged, a large number of pyramidal cells died, hippocampal apoptotic cells increased, and the expression of serum inflammatory factors IL-1β, IL-6, TNF-α, and significantly increased (P<0.05), the expression of p-PI3K, Akt, and caspase-3 in the brain tissue was significantly increased, and FoxO1 protein was decreased. There was a statistical difference between the two groups (P<0.05); compared with the I/R group, the HRS group Inflammatory factors were significantly reduced; the expression levels of p-PI3K, Akt, and caspase-3 were significantly reduced, and FoxO1 protein was increased (P<0.05).

　　Conclusion: Hydrogen-rich liquid can reduce brain damage caused by ischemia-reperfusion, and its mechanism may be related to the PI3K/Akt/FoxO1 signaling pathway.