II型糖尿病 z-bo 2020-11-17 327

　　Objective: To observe the protective effect of GDF11 on myocardial injury and the changes of myocardial apoptosis in type 2 diabetic C57BL/6J mice.

　　Method: 60 male C57BL/6J mice weighing 20-25 g were randomly divided into 3 groups: normal control group (control), type 2 diabetes model group (DM) and GDF11 intervention group (DM+GDF11). After being fed with high-fat and high-sugar feed for 4 weeks, intraperitoneal injections of 60 mg/kg streptozotocin (STZ) were carried out for 3 consecutive times to establish a type 2 diabetes mouse model. Heart function was detected, myocardial tissue was taken, TUNEL staining was used to detect the apoptotic ratio of myocardial tissue, and the expression of apoptosis-related proteins cleaved-caspase-3, Bcl-2 and Bax was detected by Western blot.

　　Result: Diabetic injury significantly down-regulates the left ventricular ejection fraction and left ventricular short axis shortening rate, and increases the myocardial apoptosis rate. The administration of recombinant GDF11 protein can significantly improve cardiac function and reduce myocardial apoptosis.

　　Conclusion: Exogenous GDF11 can significantly reduce myocardial apoptosis after diabetic injury and improve cardiac function.