2型糖尿病 z-bo 2020-11-17 347

　　Purpose: To compare the ulcer wound healing characteristics of C57BL/6J-db/db and STZ-induced C57BL/6J mice with spontaneous mutations of genes commonly used in type 2 diabetic mice, and to conduct related animal experiments for the application of stable diabetic mouse ulcer models Provide evidence.

　　Methods: The ulcer model of diabetic mice was established, and the dynamic healing rate and healing time of wounds in mice at 0, 3, 5, 7, 10, and 14 days were counted; tissues were taken at 7, 14 days, and HE staining and Masson staining, immunohistochemistry (CD31 And PCNA) to observe the pathological changes of the wound; fluorescence quantitative determination of the gene expression of healing-related factors collagen-Ⅲα and α-SMA.

　　Results: The wound healing time of genetic mutation db/db mice was significantly delayed compared with STZ-induced mice, from (16.6±0.8) days to (20.2±1.3) days (P<0.001); the db/db group was more granular than the STZ group Slow tissue growth, insufficient neo-epithelial length, disordered collagen deposition, and poor wound healing; CD31 and PCNA in the db/db group were significantly low expression (P<0.01); 7, 14 days, the db/db group genes were up-regulated The multiple of the amount was significantly lower than the STZ group.

　　Conclusion: Both diabetic mice have delayed wound healing, but the genetic mutation diabetic db/db mice are more suitable for diabetic ulcer wounds in terms of delayed wound healing and difficulty of healing compared with STZ induced mice Research.