动物实验 z-bo 2020-11-17 378

　　Purpose: The delayed response test based on the Wisconsin Universal Tester is a common method to evaluate the learning and memory ability of non-human primates. The experiment is affected by many factors during the process. How to set standard training and evaluation parameters to correctly evaluate monkeys The ability of learning and memory has important reference significance and value.

　　Methods: In this study, male cynomolgus monkeys aged 8-9 years were selected as the experimental research object, and the improved Wisconsin general tester was used to evaluate the training times, evaluation frequency, interval evaluation time, and delay time through the correct rate of food obtained by cynomolgus monkeys The influence of experimental factors such as the number of traps and food boxes on the results.

　　Results: When the number of training sessions is reduced (less than 10 times), the correct rate of cynomolgus monkeys obtaining food is unstable and shows a significant downward trend; on the premise of sufficient training, the evaluation frequency of cynomolgus monkeys obtaining food The accuracy rate of cynomolgus monkeys has little impact; evaluation 3 months after regular training, cynomolgus monkeys can still obtain 65% of the correct rate of obtaining food; increasing the delay time will result in a significant decrease in the accuracy of some cynomolgus monkeys obtaining food; , When increased to more than 3 trap food boxes, the correct rate of obtaining food for cynomolgus monkeys is generally lower than 65%.

　　Conclusion: The reasonable setting of experimental parameters in the training and evaluation phase has important influence and significance on the evaluation of the learning and memory function of non-human primates.