(1) P3 laboratory operation specifications

　　1. In order to reduce the occurrence of accidents, it is necessary to limit the number of people who can enter the laboratory at the same time.

　　2. Before starting work, please make sure that the air flows into the laboratory and the laboratory is under negative pressure.

　　3. If you use EPC (exhaust protection cabinet), please ensure that the air flow direction is correct.

　　4. Make sure you have enough supplies and disinfectants.

　　5. All operating procedures for infectious substances must be performed on the EPC, and the EPC work surface must be disinfected after each work.

　　6. All discarded laboratory materials should be disposed of in disinfectant or autoclaved buckets.

　　7. If the transmitted infectious agent is used, please put it in a sealed centrifuge bucket.

　　8. The centrifuge bucket can only be opened in EPC.

　　9. The surface of the centrifuge must be disinfected after use.

　　10. If the centrifuge barrel needs to be balanced, it must be done on the EPC.

　　11. If you suspect that the centrifuge is damaged, you should wait 30 minutes to stop the rotation, and then perform disinfection.

　　12. Tissue culture dishes should be cultured in plastic dishes.

　　13. Culture flasks and dishes cannot be stacked.

　　14. Put the aspirated tissue culture fluid in a bucket containing a certain concentration of disinfectant.

　　15. When taking out the glass bottle from the liquid nitrogen, please be careful not to directly touch the liquid nitrogen and be careful not to explode.

　　16. All items taken out of the laboratory should be surface disinfected, unless they are placed in their own transport container.

　　17. When dealing with large-scale infection sources, please discuss with the person in charge of the laboratory and strengthen preventive measures as necessary.

　　18. Overtime work requires consent, such as notification to safety officials and regular reports. It can be used with personal alarms or avoid working alone. In addition, the entire laboratory should be visible in the observation window.

　　19. If conditions permit, the computer will be on duty and use the staff’s special computer identification card to enter the laboratory for recording and archiving.

　　(two) P3 laboratory operation specifications

　　1. All personnel entering the infected animal testing area must wear protective clothing, rubber boots, masks and glasses, and gloves (double gloves are allowed).

　　2. After the work is completed, the personnel will wash and disinfect their hands before leaving the scene. Gloves, masks and clothing should be autoclaved for future use.

　　3. Change toilets and catch animals to prevent dust from spreading and reduce aerosol production.

　　4. Before cleaning, the used cages and laboratory equipment must be autoclaved and disinfected.

　　5. Animal feces, debris and residual feed should be thoroughly disinfected and sterilized (preferably high temperature incineration) before processing.

　　6. Dead animals must be autoclaved before being sealed and packaged and incinerated.

　　7. Strictly restrict the entry of persons unrelated to animal testing.

　　8. The infected animal is best raised by the experimenter.

　　9. The infected animals are stored in a negative pressure laminar flow rack or a special exhaust protection cabinet.

　　10. Experimental treatment or dissection should be performed on a negative pressure ultra-clean workbench or on an infected animal in an isolation cover, and placed in a tray to prevent the skin and liquid from spreading everywhere. After the experiment is completed, disinfect and incinerate.

　　11. The electric steam autoclave is installed in the infected animal testing area, and it can disinfect various items at any time.

　　(3) Use and maintenance of EPC

　　1, EPC use program

　　(1) EPC is only available when the blower is on and the airflow indicator is in a safe position.

　　(2) If the glass panel of the EPC can be opened or closed, do not open it while working.

　　(3) The equipment and materials used should be kept to a minimum and placed in a principle that does not block the air flow.

　　(4) Do not use the centrifuge with Type I and Type II EPC.

　　(5) After completion, all equipment and container surfaces should be disinfected and then removed from the EPC. During this operation, the EPC blower should keep running.

　　(6) Gas cannot be used for EPC.

　　(7) Type I and Type II EPC cannot prevent accidental overflow damage, nor can it avoid personal injury caused by improper operation.

　　(8) After completing the EPC work, the EPC blower should run for at least 5 minutes.

　　(9) After work, the surface of the workbench must be thoroughly disinfected.

　　2, maintenance and inspection

　　(1) Check the air volume regularly (usually every week) with an anemometer (air flow meter).

　　(2) Check the readings of the five airflow indicators.

　　(3) The EPC engineer should keep the wind speed at these 5 positions within 0.7 to 1.0m/s or at a preset level.

　　(4) Perform filter integrity and operator protection tests every 6 to 12 months.