东莨菪碱致记忆障碍 z-bo 2020-11-18 341

　　Objective: To establish a memory disorder model of scopolamine and use the traditional Chinese medicine compound Golden Thinking to intervene to observe the effect of Golden Thinking on the behavior and cholinergic system of mice with memory impairment caused by scopolamine, and to explore the neuroprotective mechanism of the drug.

　　Methods: ICR mice were randomly divided into normal control group; model group, vehicle 0.5% CMC; positive control group, donepezil, 0.92 mg/ (kg·d); Jin Sidai? Medium? Low dose group, 20? 10? 5 mg/(kg·d)? 18 mice in each group, given by 0.1 mL/10 g mouse body weight continuously for 30 days? After the last administration, the model was established, the control group was injected with normal saline intraperitoneally, and the other groups were injected intraperitoneally Scopolamine 3 mg/(kg·d), dissolved in 0.9% normal saline, and injected at 0.1 mL/10 g of mouse body weight to perform Morris water maze experiment. After the experiment, the cortex and hippocampus were taken, and the acetylcholine in the cortex and hippocampus were determined. (Ach) content? Acetylcholinesterase (AchE) and choline acetyltransferase (ChAT) activity?

　　Results: Golden Thinking can shorten the swimming distance and swimming time of model mice and increase the staying time in the target quadrant; Golden Thinking can increase the Ach content in the brain of model mice? AchE activity decreases and ChAT activity increases?

　　Conclusion: Jin Siwei can improve the learning and memory ability of mice with memory impairment caused by scopolamine. The mechanism may be related to the cholinergic system?