2型糖尿病 z-bo 2020-11-18 315

　　Objective: To explore the effect of different high-fat diet feeding time on the blood glucose, insulin resistance index (HOMA-IR) and urinary albumin excretion rate (UAER) of induced type 2 diabetic nephropathy (DN) rat model?

　　Methods: Unilateral renal artery ligation + high-fat feed feeding + low-dose streptozotocin (STZ) intraperitoneal injection was used to create the model. After unilateral renal artery ligation, DN1 group and DN2 group were fed with high-fat feed 4 After 4 weeks or 8 weeks, STZ 30 mg/kg was injected intraperitoneally. The UAER of the two groups of rats after 4 weeks of STZ injection and at the end of the experiment were compared, and the blood glucose, body weight, HOMA-IR, renal index and Kidney pathology, etc.?

　　Results: 4 weeks after STZ injection, both groups of rats could have an increase in UAER, and the UAER of DN2 group was significantly higher than that of DN1 group (P<0.01)? At the end of the 12th week, HE staining of kidney tissues in DN1 and DN2 groups were both visible The glomerular capillary loops are hypertrophy and the mesangial matrix increases; pathological changes such as renal small sac cavity stenosis? The body weight of the DN2 group was higher than that of the DN1 group (P <0.01), and the renal index was lower than that of the DN1 group (P <0.01). serum="" insulin="" homa-ir="" and="" uaer="" were="" not="" significantly="" different="" from="" those="" in="" the="" dn1="" group="" p="">0.05)?

　　Conclusion: Extending the feeding time of high-fat feed can aggravate DN kidney damage, but it may delay the intervention time, so the choice should be weighed in the trial?