A型肉毒素重链,大鼠脊髓损伤 z-bo 2020-11-18 377

　　OBJECTIVE: To investigate the effect of artificially recombined botulinum toxin type A heavy chain (BoNT/A heavy chain) on the expression of local growth-related proteins of spinal cord injury in vivo in rats, and provide evidence for elucidating the mechanism of BoNT/A heavy chain promoting neurite regeneration.

　　Methods: A rat unilateral lumbar spinal cord transection injury model was established and BoNT/A heavy chain was administered locally and intrathecally; the total protein expression was detected by two-dimensional electrophoresis on local bone marrow tissues at different times after treatment; growth-related protein-43 (GAP- 43) and superior cervical ganglion protein 10 (SCG 10) were detected by Western blot and immunofluorescence to observe their expression and distribution under the influence of BoNT/A heavy chain.

　　Results: (1) Unilateral lumbar spinal cord injury model animals showed obvious unilateral motor and sensory dysfunction; (2) Administration of BoNT/A heavy chain after spinal cord injury can affect the local protein expression profile changes: BoNT/A heavy chain It can cause the damage of local protein spots to reverse or increase their expression on the basis of changes, especially with MW between 35 ~ 45 kDa and 18 ~ 25 kDa. Protein spots with an isoelectric point in the range of 5-7 are obvious; (3) Western blot and immunofluorescence staining results indicate that BoNT/A heavy chain can promote the expression of p-GAP 43 and SCG 10 (P<0.05), and p -GAP 43 and SCG 10 are mainly positive for cells around the injury, and both cytoplasm and protrusions are positive.

　　Conclusion: BoNT/A heavy chain administration after spinal cord injury can promote the expression of selective growth-related proteins p-GAP 43 and SCG 10 after spinal cord injury.