小鼠造血干细胞 z-bo 2020-11-18 479

　　Objective: To detect the effects of different administration methods on the function of hematopoietic stem cells in mice.

　　Methods: C57 mice were given busulfan intraperitoneally, divided into high-dose group (40 mg/kg) and low-dose group (20 mg/kg), the low-dose group was given once, and the high-dose group was given for two days Administration, 20 mg/kg per day. Detect the mouse peripheral blood and bone marrow cell counts, the proportion of hematopoietic stem cells, hematopoietic progenitor cells and long-term hematopoietic stem cells 15 d and 30 d after administration, respectively, evaluate the proliferation ability of mouse hematopoietic progenitor cells through the CFU-GM experiment, and flow sorting for long-term Hematopoietic stem cells are cultured in vitro, and the function of hematopoietic stem cells is tested by single cell colony experiment.

　　Result: Both the high-dose group and the low-dose group of busulfan can reduce the number of white blood cells and platelets in the peripheral blood of C57 mice, reduce the ratio of hematopoietic stem progenitor cells and long-term hematopoietic stem cells, and reduce CFU-GM and single cell colony forming ability. The injury effect of the high-dose group was more severe than that of the low-dose group. There was no significant difference between the weight of mice and the control group.

　　Conclusion: The high-dose group of Busulfan can induce hematopoietic stem cell damage after 15 days, which provides a model and basis for the study of hematopoietic stem cell damage mechanisms and damage protection.