红细胞生成素,缺氧缺血性脑病 z-bo 2020-11-19 360

　　Objective: To make a hypoxic-ischemic encephalopathy (hyPoxic?ischemic encePhaloPathy, HIE) model in neonatal rats to study the brain protection of recombinant human erythropoietin (EPO) and the diversity of HIE intestinal flora in neonatal rats Changes, provide experimental evidence for the clinical application of EPO in the treatment of neonatal hypoxic-ischemic encephalopathy?

　　Methods: Seven-day-old SD rats were selected to make HIE models, and they were randomly divided into HIE model group, EPO experimental group, and control group. The expression of nestin was observed by immunohistochemistry. At the same time, the feces of each group were collected and used 16s rRNA sequencing method to observe the changes of intestinal flora?

　　Results: The expression levels of nestin in each group of rats at the same time point were significantly different (P<0.05), the control group was the lowest, the EPO experimental group was the highest, and the HIE model group was followed by the Shannon Wiener index of the HIE model group. index) Compared with the control group?

　　Conclusion: Exogenous administration of EPO can promote the growth of nerve cells in the HIE neonatal rat model and has a certain protective effect. At the same time, the diversity of the intestinal flora of rats has changed?