大鼠门静脉高压 z-bo 2020-11-19 329

　　Objective: To study the effect of simple portal hypertension on serum endotoxin and intestinal mucosa in rats?

　　Methods: Sixteen rats were selected and divided into a blank control group (4 rats) and a model group (3 d group? 7 d group? 10 d group, 4 rats in each group). The model group constructed partial portal vein ligation models, respectively. 3 d to 7 d to 10 d blood and rat jejunum, ileum and colon were collected, the serum endotoxin content was detected by ELISA method to observe the changes of endotoxin, and the intestinal tissues of each group were collected for HE staining and histopathology Observation analysis?

　　Results: All rats in the model group successfully constructed a partial portal vein ligation model? The results of serum endotoxin at three different time points were not significantly different from those of the normal control group. After 3 days of modeling, no significant changes occurred in each intestinal segment. Injury; 7 days after molding, each intestinal segment showed partial destruction and swelling of the local intestinal villi of the mucosal layer on the 3rd day, and the connection between epithelial cells and the lamina propria appeared loose; 10 days after molding was compared with 3 d and 7 d Intestinal mucosal layer and lamina propria have obvious damage?

　　Conclusion: In the case of normal liver function, portal hypertension can cause intestinal mucosal damage in a short period of time, but the endotoxin produced in the intestine will not exceed the processing capacity of the liver and increase in serum endotoxin?