体外染色体畸变 z-bo 2020-11-19 591

　　Objective: To discuss the influencing factors and quality control of the chromosome preparation process in the in vitro chromosome aberration test, and to summarize and analyze the successful preparation methods and main points of in vitro chromosome specimens.

　　Method: Use Chinese hamster lung cells (CHL) for cell culture and chromosome preparation, and use mitomycin and cyclophosphamide as the positive mutagens. After routine hypotonicity, fixation, droplets, and finally microscopic review.

　　Results: In the CHL chromosome aberration test, the chromosome aberration rate was significantly increased after treatment with mitomycin and cyclophosphamide, all >20%; while the negative control group had both the chromosome structure aberration rate under both test conditions with and without metabolic activation system Less than 5%. The prepared chromosomes are well dispersed, the length is moderate, and the success rate is high.

　　Conclusion: There are many factors that affect the preparation of chromosome specimens for in vitro chromosome aberration tests. Each step is critical. You must strictly follow the operating procedures, master the principles of each step, and operate carefully, patiently, and scientifically to prepare good specimens.