放射性肺损伤 z-bo 2020-11-20 517

　　Objective: To explore the protective effect of mild hypothermia treatment on radiation-induced lung injury, and compare the protective effect with the clinically commonly used anti-radiation drug Amifostine.

　　Methods: 75 male SD rats were randomly divided into a blank control group, a simple irradiation group, an amifostine positive control group, a mild hypothermia prevention group, and a mild hypothermia treatment group. Except the blank control group, the rest of the groups received 20Gy electronic beam full chest For a single irradiation, the amifostine-positive group was intraperitoneally injected with amifostine 30 minutes before irradiation; the rats in the mild hypothermia prevention group were irradiated with radiation after their body temperature fell to a mild hypothermia state, and maintained for 6 hours; the rats in the mild hypothermia treatment group were irradiated The body temperature was maintained at a mild hypothermia state for 6 hours. On the day after the irradiation, the lung histopathological examination and the collagen fiber examination were performed on the 14th and 35th days, and the content of malondialdehyde (MDA) in the rat serum was measured, and the reduced form of the valley Glutathione (GSH) content and superoxide dismutase activity (SOD), detect TNF-α content in lung tissue homogenate, and observe lung tissue apoptosis.

　　Results: The SOD activity of each irradiated group decreased immediately after irradiation. On the 14th day, the SOD activity of the mild hypothermia treatment group was higher than that of the simple irradiation group (P<0.05); compared with the simple irradiation group, the amifostine positive group and the mild hypothermia intervention group Serum MDA levels were significantly reduced on the 14th day (P <0.05); TNF-α levels in the simple irradiation group increased significantly at 6 h after irradiation, and the amifostine-positive group and mild hypothermia treatment group had a greater degree of increase compared with the simple irradiation group. Low (P<0.05); on the day of irradiation, the apoptotic cells in the simple irradiation group increased greatly and distributed widely, and each intervention group decreased correspondingly, but the difference between groups was not significant.

　　Conclusion: Mild hypothermia treatment can improve the anti-oxidation ability, anti-inflammatory ability and anti-apoptosis ability of radiation-induced lung injury rats, which is similar to the effect of the commonly used clinical drug amifostine.