铁皮石斛多糖,慢性萎缩性胃炎 z-bo 2020-11-20 378

　　Objective: To investigate the effect of Dendrobium officinale polysaccharide on chronic atrophic gastritis (CAG) induced by nitrosoguanidine (MNNG) in rats and its molecular mechanism.

　　Methods: 120 rats were randomly divided into normal group, model group, Yangweishu group, Dendrobium officinale polysaccharide high, medium, and low dose group, 20 per group. Model group, Yangweishu group, Dendrobium officinale polysaccharide high, medium ､The low-dose group was given 167μg/mL MNNG drinking solution, and the normal group was free to drink distilled water for a total of 8 weeks. After the model was successfully built, the high-dose Dendrobium officinale polysaccharide group was given a daily dose of 1.34g/kg of Dendrobium officinale polysaccharide extract crude drug ; Medium-dose group 0.67g/kg; low-dose group 0.335g/kg. Yangweishu group was given Yangweishu granules 4.0g/kg daily by gavage. The model group was given normal saline by gavage, and the normal group was given no intervention. Give After 12 weeks of treatment, the gastric tissue was taken out for histopathology, gastrin (Gas), interleukin-6 (IL-6), proliferating Cell Nuclear Antigen (PCNA), tumor necrosis factor-α (tumornecrosis factor- α,TNF-α mRNA), Janus kinase 2 (JAK2), activator-3/tyrosine phosphorylation 3 (p-STAT3/STAT3) detection. Results After the 12th week of treatment, the normal group had high polysaccharides from Dendrobium candidum The body weight of the medium and low-dose group and the Yangweishu group was higher than that of the model group (P<0.05). The weight of the Dendrobium officinale polysaccharide high and medium-dose group was higher than that of the Yangweishu group (P<0.05); ②The inflammation grade of the model group Higher than the normal group (P<0.01), while the number of glands, thickness of glands, number of principal cells, and parietal cells is lower than the model group (P<0.05). Dendrobium officinale polysaccharide high, medium, and low dose group, nourishing the stomach Inflammation grade, number of glands, gland thickness, number of principal cells, and number of parietal cells in the group were recovered compared with the model group (P<0.05). Compared with the Yangweishu group, the main cells of the Dendrobium officinale polysaccharide high and medium dose group Increase in number (P<0.05); the number of parietal cells in the high-dose Dendrobium officinale polysaccharide group increased (P<0.05); ③In the normal group, the high, medium, and low-dose Dendrobium officinale polysaccharide group, the serum GAS content of the Yangweishu group was higher than Model group (P<0.05). In the normal group, the high-dose Dendrobium candidum polysaccharide group had lower serum IL-6 levels than the model group (P<0.05); ④The normal group, the Dendrobium candidum polysaccharide high-dose and medium-dose group had PCNA and TNF-. αmRNA was lower than the model group (P<0.05); ⑤The protein expression of the normal group, Dendrobium officinale polysaccharide high and medium dose group JAK2, p-STAT3/STAT3 were lower than the model group (P<0.05). Conclusion Dendrobium officinale polysaccharide on CAG Rats have good protective and reversal effects, which can effectively restore weight and relieve gastric mucosal atrophy. The mechanism of action may be to inhibit the activation of JAK2/p-STAT3 signaling pathway to improve the pathological state of gastric mucosa.