脾肾阳虚型UC z-bo 2020-11-21 347

　　Objective: To find the specific target of UC of spleen and kidney yang deficiency type.

　　Methods: 96 Wistar rats were randomly divided into model group, high-dose group, medium-dose group, low-dose group, SASP group, and the treatment group was given the corresponding drug gavage treatment. The colon tissue of the blank group and the model group were selected respectively High-throughput sequencing of the colon tissue at the lesion site. RT-qPCR method to detect the gene expression of the selected chemokines.

　　Results: Compared with rats in the model group, the differentially expressed genes in the blank group were screened according to q-value≦0.0 fold-change≧1.5. The GO functional classification analysis showed that the differential gene function was mainly enriched in biological process (biological process). ,BP), cellular component (CC), molecular function (molecular function, MF) three levels. Enrichment analysis of differential genes KEGG found that CXCL1, CXCL2, CXCR2, CXCL6, CCL7, CCL12 gene expression in the chemokine signaling pathway Significantly up-regulated; and verified by RT-qPCR method, the gene expression changes of the above factors are consistent with the sequencing results. After the treatment of warming the spleen and kidney, the expression of the above factors was significantly down-regulated.

　　Conclusion: The expression of CXCL1, CXCL2, CXCR2, CXCL6, CCL7, and CCL12 genes in the chemokine signaling pathway of spleen and kidney yang deficiency ulcerative colitis is significantly up-regulated, which can be used as an objective indicator of UC mucosal inflammation. It has the effect of warming the spleen and kidney. Tang and Si Shen Wan compound Chinese medicine particles can effectively down-regulate the expression of the above factors, slow down the inflammatory response, and promote the repair of the injured colon mucosa.