姜黄素,肾病理损伤 z-bo 2020-11-21 346

　　OBJECTIVE: To observe the effects of curcumin pretreatment on renal pathological changes and oxidative stress 9 levels in kidney tissue in rats with different degrees of heatstroke. Methods 80 healthy male SD rats were randomly divided into 2 groups (n=40): saline control group And curcumin pretreatment group. Each group is divided into 4 subgroups (n=10): dry heat 0min group (ie normal temperature group), dry heat 50min group (mild heat stroke group), dry heat 100min group (moderate Heatstroke group), dry heat 150min group (severe heatstroke group). Rats in saline group were given 0.9% saline by gavage, rats in curcumin group were given 200mg/kg curcumin by gavage; rats in each group were given continuous gavage The 7d｡0min group was placed in a normal temperature environment, and the rest of the groups were placed in the artificial experiment cabin in the northwest special environment. The dry heat environment temperature was set: (41±0.5)℃, humidity (10±1)%, and the anesthesia at the corresponding time point Rats, take blood and kidney tissue. Detect blood creatinine and urea nitrogen content; make kidney tissue homogenate for superoxide dismutase, catalase and malondialdehyde activity Detection; make a slice of kidney tissue, HE stain, observe pathological changes.

　　Results: Pathological examination found that the pathological changes and scores of kidney tissues aggravated with the extension of dry heat environment. At the same time point, the scores of renal pathological damage in the curcumin pretreatment group were significantly lower than those in the saline group (P<0.05, P <0.01); Serum creatinine, urea and MDA content in kidney tissue were significantly lower in the curcumin group than in the saline group in the three heatstroke stages (P<0.05); the activity of antioxidant enzyme SOD and CAT in the kidney tissue was in the curcumin group Both were significantly higher than those in the saline group (P<0.05). Correlation analysis indicated that MDA was closely related to the pathological damage score (r=0.75, P<0.01).

　　Conclusion: Curcumin pretreatment has a protective effect on kidney damage in rats with different degrees of heatstroke in a dry heat environment, which may be partly achieved by anti-oxidative stress response.