参一胶囊联合5-氟尿嘧啶,结肠癌 z-bo 2020-11-21 376

　　Objective: To explore the inhibitory effect of Shenyi capsule combined with 5-fluorouracil (5-fluorouracil, 5-FU) on colon cancer growth and angiogenesis and its possible mechanism.

　　Methods: Fifty BALB/c mouse LoVo colon cancer models were established, and they were randomly divided into 5 groups. According to different treatment methods, they were divided into model group, 5-FU group, Shenyi capsule low-dose group, Shenyi capsule high-dose group, and Shenyi Capsule + 5-FU group. Detect and record the tumor volume, tumor microvessel density (MVD) and immune organ changes in each group of mice, and use Western-Blot method to detect tumor Gli1 and VEGF protein expression.

　　Results: 1) Compared with the model group, the tumor volume of mice in each treatment group was significantly suppressed. The high-dose Shenyi Capsule group and the Shenyi Capsule+5-Fu group had the highest tumor inhibition rate, and with the time of administration (2) Compared with the model group, the immune organ quality and index of mice in the 5-FU group decreased, and the other treatment groups improved to varying degrees. Shenyi Capsule+5-FU Thymus quality, thymus index, spleen quality, and spleen index improved most significantly in the group, which was significantly better than all other treatment groups (P<0.05). p="">0.05). High-dose Shenyi Capsule Group, Shenyi Capsule +5 The metastasis rate of colon cancer in all organs of mice in the -FU group was significantly reduced (P<0.05). p="">0.05). Gli1 protein and VEGF protein in Shenyi capsule high-dose group, Shenyi capsule + 5-FU group The expression level was significantly reduced (P<0.05).

　　Conclusion: Shenyi Capsule has a significant inhibitory effect on the growth and angiogenesis of colon cancer cells. This inhibitory effect is more significant when 5-FU is used in combination. The expression of VEGF related to the Hedgeog-Glin1 signaling pathway may be the main factor. Action target.