胚胎发育 z-bo 2020-11-21 360

　　Objective: To investigate the effects of different doses of perfluorooctane sulphonate (PFOS) on rat embryo development and the expression of insulin-like growth factor IGF-1 (insulin-like growth factor-1) in the placenta.

　　Methods: Forty-eight SD female rats on the 12th day of pregnancy were randomly divided into 4 groups and given different doses of PFOS (0, 5, 10, 20 mg/kg), continuously gavage for 7 days, and the pregnant rats were killed on the 19th day of pregnancy. Body mass of mice and fetuses, liver coefficient of fetal mice, serum PFOS and glucocorticoid (GC) levels of pregnant mice, and expression level of placental IGF-1.

　　Results: Compared with the control group, with the increase of the PFOS dose, the serum PFOS of the pregnant rats in the exposure group accumulated significantly, and the body mass of pregnant rats, fetal rats and body length, and placenta mass were gradually decreased. There was a significant difference in the PFOS20mg/kg group (P<0.001); the liver coefficient of fetal mice also showed the same trend. The low, medium and high doses of the poisoned group were significantly lower than the control group (P<0.05), and the blood biochemical alanine aminotransferase (ALT) was compared with Tianmen Aspartate aminotransferase (AST) also increased with the increase in the dose of PFOS (P<0.05); in the PFOS 10 mg/kg group, the serum GC level of pregnant rats increased (P<0.05); placental IGF- 1 The expression level decreases with increasing PFOS dose.

　　Conclusion: Long-term exposure to PFOS during pregnancy will cause the accumulation of serum PFOS content in pregnant mice, which will lead to fetal mouse liver toxicity, increase GC content, reduce placental IGF-1 expression, and ultimately affect the growth and development of fetal mice.