尿毒症大鼠 z-bo 2020-11-23 314

　　Objective: To compare the application of two hemostasis methods in rat uremia model.

　　Methods: Male SD rats were divided into two groups A and B. Both left kidney 2/3 resection and right nephrectomy were used to make uremic models. In group A, 3M tissue glue was used for hemostasis during left kidney 2/3 resection, and in group B, electric Blood coagulation and hemostasis, intraoperative blood loss and left renal blood flow blocking time, postoperative comparison of rat survival rate, measurement of creatinine value, to achieve 2 to 3 times the normal blood creatinine value is a uremic rat model. Kidney tissue Perform pathological examination.

　　Results: The blood creatinine of the two hemostasis methods increased as much as the requirements of the uremia model; the blood flow block time of the left kidney in group A was (59.97±7.56) s, group B was (174.1±15.28) s (P<0.05); A The bleeding volume of left nephrectomy in group was (0.11±0.037) mL, group B was (0.24±0.056) mL (P<0.05); the pathology of renal tissue in both groups showed glomerular ischemia shrinkage and tubulointerstitial fibers The postoperative survival rate of group A was significantly higher than that of group B (P<0.05).

　　Conclusion: 3M tissue glue is superior to electrocoagulation to stop bleeding whether it is bleeding time, bleeding volume, or postoperative survival rate.