小鼠脑缺血/再灌注损伤,二苯乙烯苷 z-bo 2020-11-23 379

　　Objective: To investigate the protective effect of stilbene glycosides (TSG) inhibiting NADPH oxidase on cerebral ischemia-reperfusion (I/R) injury in mice.

　　Method: 100 experimental mice were randomly divided into 5 groups: sham operation group, model group, TSG low dose group (3mg/kg), TSG medium dose group (6mg/kg), TSG high dose group (12mg/kg), 20 mice in each group. Mice were cerebral ischemia for 2h caused by ligation of bilateral common carotid arteries, and the mice were sacrificed after 24h of reperfusion. The mouse brain tissue was examined by HE staining method for pathology, using DHE staining method and ESR spectrometer Detect the level of reactive oxygen species (ROS) in brain tissue, and detect the expression of NOX4 and cleaved caspase-3/9 protein in brain tissue by Western blot.

　　Results: After cerebral ischemia and reperfusion in mice, severe pathological damage occurred in the cortical brain tissue of the ischemic area, the level of ROS was significantly increased, the expression of NOX4 protein in brain tissue was significantly up-regulated, and the apoptosis-related protein cleaved caspase-3/9 Protein expression increased significantly. TSG can significantly reduce the pathological damage of ischemia-reperfusion brain tissue in mice, inhibit the production of ROS, significantly down-regulate the expression of oxidative stress protein NOX4, and significantly inhibit the apoptosis-related protein cleavedcaspase-3/9 Protein expression.

　　Conclusion: TSG can inhibit the expression of oxidative stress protein NOX4 in brain tissue, reduce the production of reactive oxygen species, and inhibit the overexpression of apoptosis-related protein cleaved caspase-3/9, and protect mice against cerebral ischemia-reperfusion injury.