2型糖尿病,造血免疫系统 z-bo 2020-11-23 755

　　Objective: To observe the damage effect of whole body X-ray irradiation on the hematopoietic immune system function of type 2 diabetes model KKAy mice, and compare it with the control C57 mice.

　　Methods: KKAy mice were divided into a control group and an irradiation group. The mice in the irradiation group were irradiated with X-rays at a dose of 4 Gy, and C57 mice were used as controls. 15 days after irradiation, the mice’s peripheral blood routine was tested, and flow cytometry was performed The ratio of hematopoietic progenitor cells, hematopoietic stem cells and long-term hematopoietic stem cells in the bone marrow, the ratio of B cells and T cells in the spleen, the ratio of CD4CD8 double-positive T cells in the thymus, the ratio of CD4 single-positive T cells and CD8 single-positive T cells. Through granulocytes The colony forming ability test evaluates the function of mouse hematopoietic progenitor cells.

　　Results: The ratio of HSC and LT-HSC in KKAy mice before irradiation was lower than that in C57 mice. After 4 Gy whole body irradiation, the peripheral blood WBC, RBC, PLT, HGB and LYM% of KKAy mice decreased by 68.42% and 12.17 respectively. %､8.78%､30.12%､70.84%; the proportion of HPC, HSC and LT-HSC in the bone marrow decreased by 34.02%, 29.49%, 35.74%; the proportion of spleen B cells and T cells decreased by 57.85%, 58.81%, respectively ; The percentage of thymus CD4CD8 double-positive cells decreased by 51.70%. The reduction of bone marrow HSC, LT-HSC, peripheral blood RBC and HGB of KKAy mice was significantly lower than that of C57 mice.

　　Conclusion: 4Gy whole body irradiation damages the hematopoietic immune system function of KKAy mice. KKAy mice may show stronger tolerance to ionizing radiation than C57 mice.