大鼠外周痛感觉 z-bo 2020-11-24 354

　　Purpose: To observe the relationship between TRPV1 and P2X3 in the periphery of rats, in order to partially clarify the mechanism of peripheral pain sensory regulation.

　　Methods: Male SD rats were randomly divided into blank control group, TRPV1 agonist group, P2X3 agonist group, TRPV1 agonist P2X3 agonist group, TPRV1 agonist P2X3 inhibitor group, P2X3 agonist TRPV1 inhibitor group, and through the sole Inject TRPV1 or P2X3 agonists and/or inhibitors subcutaneously, and observe the number of foot withdrawals and the duration of leg raising/licking in each group within 20 minutes; immunofluorescence method was used to observe the expression and co-expression of the positive area of TRPV1 and P2X3 in L4DRG Situation: Use immunoprecipitation method to observe the relationship between L4DRG levels TRPV1 and P2X3.

　　Results: P2X3 agonists can not improve the pain behavior induced by TRPV1 agonists, P2X3 inhibitors can reduce the pain behavior induced by TRPV1 agonists; TRPV1 agonists can increase the pain behavior induced by P2X3 agonists, and TRPV1 inhibitors will not reduce the pain behavior. Pain behavior induced by P2X3 agonists. P2X3 agonists can increase the expression of TRPV1 positive area at L4 DRG level, and TRPV1 agonists can increase the expression of P2X3 positive area at L4 DRG level; TRPV1 and P2X3 are co-expressed at DRG level and there is co-precipitation.

　　Conclusion: At the level of peripheral neurons, there is a certain interaction between TRPV1 and P2X3. The two can promote the expression of each other. When one of them is inhibited, the function of the other will decrease accordingly.