小鼠肺损伤,流感病毒A/PR/8/H1N1 感染 z-bo 2020-11-24 376

　　Objective: To observe the effect of a compound Chinese medicine prescription on lung injury caused by influenza virus A/PR/8/H1N1 infection in mice.

　　Methods: Ninety female BALB/c mice were randomly divided into 6 groups: blank control group, virus control group, amantadine hydrochloride group and compound Chinese medicine high, medium and low dose group, 15 mice in each group. Chinese medicine group was continuously irrigated After 2 days of stomach instillation, mice were infected with nasal drip to establish influenza virus mouse model. The blank control group was dripped with nasal saline. After 4 hours of virus infection, each group was given different doses of drugs or saline, and then continuously fed for 5 days. On the 5th day of infection Calculate the lung index of each group of BALB/c mice, measure the hemagglutination titer of lung tissue homogenate, and observe the pathomorphology of lung tissue.

　　Results: Compared with the virus control group, the lung index of the amantadine hydrochloride group and the compound Chinese medicine preparation medium-dose group was significantly lower (P<0.01), and the lung index of the low-dose compound Chinese medicine preparation group and the high-dose compound Chinese medicine preparation group was significantly lower (P <0.05); The blood coagulation titer of lung tissue of mice in each drug group was significantly reduced (P<0.01); the pathomorphology of lung tissue of mice in each drug group was improved to different degrees.

　　Conclusion: The compound Chinese medicine prescription used in this study has a significant reduction in the average lung index and average blood coagulation titer of mice infected with influenza virus A/PR/8/H1N1, and has a significant improvement effect on the lung injury caused by it.