KK鼠料快速建模 z-bo 2020-11-24 409

　　Objective: To study the rapid modeling of KK rodent feed and its effect on related indexes of KK/Upj-Ay/J mice.

　　Methods: The experiment was carried out with 40 female and male KK/UpjAy/J mice, divided into 4 groups, namely the normal feed group (female and male each 10), KK rat feed group (female and male 10 each) ､Normal fasting blood glucose group (10 females and 10 males), KK rat fasting blood glucose group (10 females and 10 males). Feeding from 3 weeks until the end of the experiment at 23 weeks. Weight and fasting blood glucose are measured weekly After the experiment, serum was collected to detect biochemical indicators, and the pancreas, kidneys, and livers were fixed for HE and special staining (the pancreas was stained with insulin immunohistochemistry, the liver was stained with PAS, and the kidney was stained with PASM).

　　Results: KK rat feed can significantly promote the weight gain of KK/Upj-Ay/J mice, increase the fasting blood glucose level of KK/Upj-Ay/J mice, and increase the alanine aminotransferase in the serum of KK/Upj-Ay/J mice Content (P<0.05). KK rat feed can significantly increase the serum creatinine content of female KK/Upj-Ay/J mice (P<0.05), and significantly increase the serum cholesterol content of male KK/Upj-Ay/J mice ( P<0.05), but it has no significant effect on the serum cholesterol content of female KK/Upj-Ay/J mice. The pathological changes of the pancreas of female KK/Upj-Ay/J mice at the end of the feeding experiment are obvious, but the male KK/Upj -Ay/J mouse pancreas pathological changes have no obvious effect. Presumably related to the physiological characteristics of male KK/Upj-Ay/J mice. KK rat diet significantly promoted hepatocyte steatosis in KK/Upj-Ay/J mice. Feeding At the end of the feeding experiment, compared with the C57BL/6J group, the pathological changes of the kidney in the normal feed feeding group and the KK rat feeding group were mainly manifested as tubulin casts, interstitial nephritis, perivascular inflammation, and mesangial glomeruli Increased matrix. The degree of nephropathy in the KK rat feeding group was more obvious. The results of renal PASM staining showed that compared with the control group, the glomerular basement membrane of the other two groups had no dark brown material deposition, indicating that the glomerular basement membrane structure was destroyed.

　　Conclusion: KK rat feed can promote the damage of diabetic organs in KK/Upj-Ay/J mice, increase fasting blood glucose and body weight, shorten modeling time, and improve modeling uniformity.