慢性癫痫大鼠,氨基酸 z-bo 2020-11-24 361

　　Objective: To investigate the effect of lamotrigine on the expression of P-glycoprotein protein (PGP) and major vault protein (MVP) and amino acid content in hippocampus of chronic epilepsy rats.

　　Methods: SPF male SD rats were prepared with pentylenetetrazol for chronic epilepsy rats and randomly divided into model group, lamotrigine low-dose (5mg/kg) group and lamotrigine high-dose (10mg/kg) group. Take healthy rats as the control group, 15 rats in each group, all groups were given intragastric administration, the model group and the control group were given normal saline by intragastric administration, the administration volume was 1mL/100g. Record all rats before and after treatment The behavioral characteristics and body weight of the patients, the seizure time and brain waves after treatment were recorded, the expression of PGP and MVP in the hippocampus was detected by immunohistochemistry and Western blot, and the aspartate (Asp) in the hippocampus was detected by HPLC ､Glutamate (Glu) and glycine (Gly) content.

　　Results: The rats in the control group had normal activities. The rats in the chronic epilepsy model group showed reduced activity, chewing, rhythmic nodding, head and neck raised, forelimb clonic seizures, a few rats raised their tails or tailed the ground. Lamotrigine group Rats have an early head and neck upturn and forelimb clonic seizures gradually transition to erect hairs and mouth and facial muscle twitches. Rare tails or tail beats. Before treatment, compared with the control group, the model group and lamotrigine The body mass of the group was significantly reduced (P<0.05). After treatment, compared with the control group, the model group's body mass, EEG frequency, hippocampal Gly decreased significantly (P<0.05), seizure time, EEG amplitude ､Asp, Glu, PGP and MVP in the hippocampus were increased (P<0.05), compared with the model group, the body mass, EEG frequency, and Gly of the hippocampus in the lamotrigine group were significantly increased (P<0.05), seizures Time, EEG amplitude, hippocampal Asp, Glu, PGP and MVP decreased (P<0.05), and showed a dose-dependent trend of lamotrigine.

　　Conclusion: Lamotrigine treatment of chronic epilepsy is related to reducing hippocampal PGP and MVP protein and improving hippocampal amino acids.