人脐带间充质干细胞 z-bo 2020-11-24 407

　　Objective: To explore the mechanism of transplantation of human umbilical cord mesenchymal stem cells (hUCMSCs) to repair the ovarian function of premature ovarian failure (POF) rats.

　　Methods: 30 SPF-grade SD female rats were randomly selected as a control group, and the other 20 rats were established as a POF model. The model rats were divided into a model group (10) and a hUCMSCs transplantation group (10). Observe each Group rats’ follicles and ovarian tissue ultrastructure changes. ELISA was used to detect serum levels of E2, FSH, AMH, ROS, and 8-OHdG, and immunohistochemistry and Western blot were used to detect the protein changes of SOD1 and UCP2 in rats.

　　Results: HE staining showed that the number of atretic follicles increased in the model group and the control group, while the number of growing follicles decreased (all P<0.05), the number of atretic follicles decreased in the hUMSCs transplantation group and the model group, while the number of growing follicles increased (all P<0.05) 0.05); ovarian electron microscope observation results showed that after hUMSCs transplantation treatment, rat ovarian cells appeared nuclei, nuclear membrane gradually recovered, and a few organelles appeared; ELISA results showed: serum LH and FSH levels in the hUCMSCs transplantation group, ROS and 8-OHdG production Compared with the model group (all P<0.05), compared with the control group and the model group, the E2 level of the hUCMSCs transplantation group was significantly increased (P<0.05); the results of immunohistochemistry showed: compared with the control group, the model group was immunohistochemical The number of UCP-2 and SOD1 positive cells increased, while the number of UCP-2 and SOD1 positive cells in the hUCMSCs transplantation group was less than that of the model group; Western blot results showed that the expression of SOD1 and UCP-2 protein in the ovarian tissue of the model group was more significant than that in the control group Increased (P<0.05). After hUCMSCs transplantation treatment, the protein levels of SOD1 and UCP-2 were significantly lower than the model group (P<0.05).

　　Conclusion: hUMSCs transplantation can reduce the expression of SOD1 and UCP-2, thereby reducing the oxidative stress response, and ultimately achieving the role of repairing ovarian function.