大鼠胚胎发 z-bo 2020-11-25 361

　　Objective: To investigate the effects of heroin, a mental dependence, on the development of embryonic rats and the expression of Bax in the brain.

　　Method: Wistar rats after conception were randomly divided into a control group and a heroin administration group (divided into heroin low, medium and high dose groups). On the 7th day, 16, 32 and 64 mg/kg of heroin were given continuously. Heroin 9d, observe the effect of heroin on the morphological and structural development of embryonic rats, and use enzyme-linked immunosorbent assay (ELISA) to detect the expression level of Bax in embryonic brain tissue.

　　Results: Embryo observation found that the total number of live embryos in the heroin low, medium and high dose group was reduced by 27.27%, 37.12% and 48.48%, respectively, compared with the control group. In the heroin low dose group, embryonic occipital hypoplasia and encephalocele occurred in the embryo; heroin medium In the dose group, the embryonic occipital bone, parietal bone, hypoplasia, and embryonic encephalocele were obvious; in the heroin high-dose group, the embryonic occipital bone, parietal bone, and temporal bone were hypoplasia, and the embryonic encephalocele was more obvious. ELISA test found that the heroin low, medium, and high dose group embryo brain The expression level of Bax in the tissues increased by 11.41%, 47.06%, and 83.74% respectively compared with the control group, and the difference was significant (P<0.05, P<0.01); the expression level of Bax in the embryonic cerebellum tissue increased by 17.16%, 52.96 compared with the control group. % And 90.01%, the difference is significant (P<0.05, P<0.01).

　　Conclusion: Heroin, a psychotropic substance, can significantly inhibit the morphological and structural development of embryonic rats. The inhibitory effect increases with the increase of the dose of heroin administered. Its mechanism may be related to the up-regulation of Bax expression in embryonic tissues and organs induced by heroin.