大鼠心肌缺血再灌注损伤 z-bo 2020-11-25 309

　　Objective 　 To explore the protective effect and mechanism of activated protein C (APC) on myocardial ischemia-reperfusion injury in rats.

　　Methods Thirty male SD rats were randomly divided into sham operation group (10 rats), I/R group (10 rats) and APC treatment group (10 rats). The left anterior descending coronary artery was ligated for 60 minutes and reperfused for 6 hours to make myocardium. In the ischemia-reperfusion model, ELISA and immunohistochemistry were used to detect the expression levels of TNF-α, myocardial tissue MPO and ICM-1, and HE staining to detect the myocardial pathological changes in each group.

　　Results Compared with the sham operation group, the expression levels of TNF-α, MPO and ICM-1 in the I/R group were significantly increased (P<0.01). Compared with the I/R group, the TNF-α, The expression levels of MPO and ICM-1 were significantly reduced (P<0.01), and the inflammatory cell infiltration was observed under light microscope. The I/R group was the most obvious, and the APC group was significantly reduced compared with the IR group.

　　Conclusion 　 APC has a protective effect on myocardial ischemia-reperfusion injury, and its mechanism may be related to inhibiting the expression of inflammatory factors and reducing tissue inflammatory cell infiltration.